Review



primary antibodies against human abcb1  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology primary antibodies against human abcb1
    Figure 2. MDCKII cell accumulation studies showing effects of talazoparib on transport activities of (A) <t>ABCB1,</t> (B) ABCG2, and (C) ABCC1. The cells were pre-exposed to talazoparib or model inhibitors (LY335979, Ko143, and MK571, respectively) and then treated with fluorescent chemotherapeutics, daunorubicin, or mitoxantrone. After the substrate accumulation period, the cells were trypsinized, and flow cytometer was used to quantify the intracellular fluorescence. The plotted values of relative accumulation reflect a fold increase in the accumulation of the probe substrate caused by the tested compound; they are numerically expressed as ratios of relative fluorescence units (RFUs) from treated samples to RFUs of vehicle control. Model inhibitors and vehicle controls represented 100% inhibition and 0% inhibition, respectively; their values were applied during normalization of talazoparib data and calculation of its IC50 values. Statistical analysis was accomplished using one- way ANOVA followed by Dunnett’s post hoc test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 relative to control), while the presented data are means ± SD from three independent assays. DAU, daunorubicin; MTX, mitoxantrone; TAL, talazoparib.
    Primary Antibodies Against Human Abcb1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 593 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+human+abcb1/pm36430819-172-0-26?v=Santa+Cruz+Biotechnology
    Average 96 stars, based on 593 article reviews
    primary antibodies against human abcb1 - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "Talazoparib Does Not Interact with ABCB1 Transporter or Cytochrome P450s, but Modulates Multidrug Resistance Mediated by ABCC1 and ABCG2: An in Vitro and Ex Vivo Study."

    Article Title: Talazoparib Does Not Interact with ABCB1 Transporter or Cytochrome P450s, but Modulates Multidrug Resistance Mediated by ABCC1 and ABCG2: An in Vitro and Ex Vivo Study.

    Journal: International journal of molecular sciences

    doi: 10.3390/ijms232214338

    Figure 2. MDCKII cell accumulation studies showing effects of talazoparib on transport activities of (A) ABCB1, (B) ABCG2, and (C) ABCC1. The cells were pre-exposed to talazoparib or model inhibitors (LY335979, Ko143, and MK571, respectively) and then treated with fluorescent chemotherapeutics, daunorubicin, or mitoxantrone. After the substrate accumulation period, the cells were trypsinized, and flow cytometer was used to quantify the intracellular fluorescence. The plotted values of relative accumulation reflect a fold increase in the accumulation of the probe substrate caused by the tested compound; they are numerically expressed as ratios of relative fluorescence units (RFUs) from treated samples to RFUs of vehicle control. Model inhibitors and vehicle controls represented 100% inhibition and 0% inhibition, respectively; their values were applied during normalization of talazoparib data and calculation of its IC50 values. Statistical analysis was accomplished using one- way ANOVA followed by Dunnett’s post hoc test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 relative to control), while the presented data are means ± SD from three independent assays. DAU, daunorubicin; MTX, mitoxantrone; TAL, talazoparib.
    Figure Legend Snippet: Figure 2. MDCKII cell accumulation studies showing effects of talazoparib on transport activities of (A) ABCB1, (B) ABCG2, and (C) ABCC1. The cells were pre-exposed to talazoparib or model inhibitors (LY335979, Ko143, and MK571, respectively) and then treated with fluorescent chemotherapeutics, daunorubicin, or mitoxantrone. After the substrate accumulation period, the cells were trypsinized, and flow cytometer was used to quantify the intracellular fluorescence. The plotted values of relative accumulation reflect a fold increase in the accumulation of the probe substrate caused by the tested compound; they are numerically expressed as ratios of relative fluorescence units (RFUs) from treated samples to RFUs of vehicle control. Model inhibitors and vehicle controls represented 100% inhibition and 0% inhibition, respectively; their values were applied during normalization of talazoparib data and calculation of its IC50 values. Statistical analysis was accomplished using one- way ANOVA followed by Dunnett’s post hoc test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 relative to control), while the presented data are means ± SD from three independent assays. DAU, daunorubicin; MTX, mitoxantrone; TAL, talazoparib.

    Techniques Used: Cytometry, Control, Inhibition

    Figure 9. The effect of talazoparib treatment on the ABCB1, ABCC1, and ABCG2 mRNA levels in breast cancer MCF-7 model. (A) The MTT viability assay performed after the 48 h incubation with a tested drug. (B) Gene induction studies. The MCF-7 cells were treated with talazoparib (0.5 µM) or rifampicin (25 µM). The qRT-PCR technique was employed for the assessment of the target genes’ mRNA expressions after 24 and 48 h incubation interval. The dotted lines draw the boundaries for downregulation (lower line) and upregulation (upper line) according to the EMA’s DDI-testing guidelines [24]. The plotted data are means ± SD from three independent repetitions. RIF, rifampicin; TAL, talazoparib.
    Figure Legend Snippet: Figure 9. The effect of talazoparib treatment on the ABCB1, ABCC1, and ABCG2 mRNA levels in breast cancer MCF-7 model. (A) The MTT viability assay performed after the 48 h incubation with a tested drug. (B) Gene induction studies. The MCF-7 cells were treated with talazoparib (0.5 µM) or rifampicin (25 µM). The qRT-PCR technique was employed for the assessment of the target genes’ mRNA expressions after 24 and 48 h incubation interval. The dotted lines draw the boundaries for downregulation (lower line) and upregulation (upper line) according to the EMA’s DDI-testing guidelines [24]. The plotted data are means ± SD from three independent repetitions. RIF, rifampicin; TAL, talazoparib.

    Techniques Used: MTT Viability Assay, Incubation, Quantitative RT-PCR



    Similar Products

    primary antibodies against human abcb1  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology primary antibodies against human abcb1
    Figure 2. MDCKII cell accumulation studies showing effects of talazoparib on transport activities of (A) <t>ABCB1,</t> (B) ABCG2, and (C) ABCC1. The cells were pre-exposed to talazoparib or model inhibitors (LY335979, Ko143, and MK571, respectively) and then treated with fluorescent chemotherapeutics, daunorubicin, or mitoxantrone. After the substrate accumulation period, the cells were trypsinized, and flow cytometer was used to quantify the intracellular fluorescence. The plotted values of relative accumulation reflect a fold increase in the accumulation of the probe substrate caused by the tested compound; they are numerically expressed as ratios of relative fluorescence units (RFUs) from treated samples to RFUs of vehicle control. Model inhibitors and vehicle controls represented 100% inhibition and 0% inhibition, respectively; their values were applied during normalization of talazoparib data and calculation of its IC50 values. Statistical analysis was accomplished using one- way ANOVA followed by Dunnett’s post hoc test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 relative to control), while the presented data are means ± SD from three independent assays. DAU, daunorubicin; MTX, mitoxantrone; TAL, talazoparib.
    Primary Antibodies Against Human Abcb1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+human+abcb1/pm36430819-172-0-26?v=Santa+Cruz+Biotechnology
    Average 96 stars, based on 1 article reviews
    primary antibodies against human abcb1 - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 2. MDCKII cell accumulation studies showing effects of talazoparib on transport activities of (A) ABCB1, (B) ABCG2, and (C) ABCC1. The cells were pre-exposed to talazoparib or model inhibitors (LY335979, Ko143, and MK571, respectively) and then treated with fluorescent chemotherapeutics, daunorubicin, or mitoxantrone. After the substrate accumulation period, the cells were trypsinized, and flow cytometer was used to quantify the intracellular fluorescence. The plotted values of relative accumulation reflect a fold increase in the accumulation of the probe substrate caused by the tested compound; they are numerically expressed as ratios of relative fluorescence units (RFUs) from treated samples to RFUs of vehicle control. Model inhibitors and vehicle controls represented 100% inhibition and 0% inhibition, respectively; their values were applied during normalization of talazoparib data and calculation of its IC50 values. Statistical analysis was accomplished using one- way ANOVA followed by Dunnett’s post hoc test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 relative to control), while the presented data are means ± SD from three independent assays. DAU, daunorubicin; MTX, mitoxantrone; TAL, talazoparib.

    Journal: International journal of molecular sciences

    Article Title: Talazoparib Does Not Interact with ABCB1 Transporter or Cytochrome P450s, but Modulates Multidrug Resistance Mediated by ABCC1 and ABCG2: An in Vitro and Ex Vivo Study.

    doi: 10.3390/ijms232214338

    Figure Lengend Snippet: Figure 2. MDCKII cell accumulation studies showing effects of talazoparib on transport activities of (A) ABCB1, (B) ABCG2, and (C) ABCC1. The cells were pre-exposed to talazoparib or model inhibitors (LY335979, Ko143, and MK571, respectively) and then treated with fluorescent chemotherapeutics, daunorubicin, or mitoxantrone. After the substrate accumulation period, the cells were trypsinized, and flow cytometer was used to quantify the intracellular fluorescence. The plotted values of relative accumulation reflect a fold increase in the accumulation of the probe substrate caused by the tested compound; they are numerically expressed as ratios of relative fluorescence units (RFUs) from treated samples to RFUs of vehicle control. Model inhibitors and vehicle controls represented 100% inhibition and 0% inhibition, respectively; their values were applied during normalization of talazoparib data and calculation of its IC50 values. Statistical analysis was accomplished using one- way ANOVA followed by Dunnett’s post hoc test (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 relative to control), while the presented data are means ± SD from three independent assays. DAU, daunorubicin; MTX, mitoxantrone; TAL, talazoparib.

    Article Snippet: Primary antibodies against human ABCB1 (cat. no. sc-13131), ABCG2 (cat. no. sc-377176), ABCC1 (cat. no. sc-18835), and secondary antimouse antibody (cat. no. sc-516102) were bought from Santa Cruz Biotechnology (Dallas, TX, USA).

    Techniques: Cytometry, Control, Inhibition

    Figure 9. The effect of talazoparib treatment on the ABCB1, ABCC1, and ABCG2 mRNA levels in breast cancer MCF-7 model. (A) The MTT viability assay performed after the 48 h incubation with a tested drug. (B) Gene induction studies. The MCF-7 cells were treated with talazoparib (0.5 µM) or rifampicin (25 µM). The qRT-PCR technique was employed for the assessment of the target genes’ mRNA expressions after 24 and 48 h incubation interval. The dotted lines draw the boundaries for downregulation (lower line) and upregulation (upper line) according to the EMA’s DDI-testing guidelines [24]. The plotted data are means ± SD from three independent repetitions. RIF, rifampicin; TAL, talazoparib.

    Journal: International journal of molecular sciences

    Article Title: Talazoparib Does Not Interact with ABCB1 Transporter or Cytochrome P450s, but Modulates Multidrug Resistance Mediated by ABCC1 and ABCG2: An in Vitro and Ex Vivo Study.

    doi: 10.3390/ijms232214338

    Figure Lengend Snippet: Figure 9. The effect of talazoparib treatment on the ABCB1, ABCC1, and ABCG2 mRNA levels in breast cancer MCF-7 model. (A) The MTT viability assay performed after the 48 h incubation with a tested drug. (B) Gene induction studies. The MCF-7 cells were treated with talazoparib (0.5 µM) or rifampicin (25 µM). The qRT-PCR technique was employed for the assessment of the target genes’ mRNA expressions after 24 and 48 h incubation interval. The dotted lines draw the boundaries for downregulation (lower line) and upregulation (upper line) according to the EMA’s DDI-testing guidelines [24]. The plotted data are means ± SD from three independent repetitions. RIF, rifampicin; TAL, talazoparib.

    Article Snippet: Primary antibodies against human ABCB1 (cat. no. sc-13131), ABCG2 (cat. no. sc-377176), ABCC1 (cat. no. sc-18835), and secondary antimouse antibody (cat. no. sc-516102) were bought from Santa Cruz Biotechnology (Dallas, TX, USA).

    Techniques: MTT Viability Assay, Incubation, Quantitative RT-PCR